RevoDx MTBC qPCR Kit

Together with other highly related bacteria, M. tuberculosis forms a complex, the Mycobacterium tuberculosis complex (MTBC), a single species as defined by DNA/DNA hybridization studies. The MTBC comprises seven members which include M. tuberculosis, the primary causative agent of human TB; M. bovis, which is responsible for bovine TB and includes the vaccine strain M. bovis BCG; M. africanum, the main causative agent of TB in West Africa; M. microti, which is a pathogen of voles and rarely infects humans; “M. canettii”, a rare MTBC strain that produces smooth and glossy colonies, with all known cases so far isolated from individuals having been to the horn of Africa; M. pinnipedii, also known as the seal bacillus. Mycobacterium tuberculosis has circular chromosomes of about 4,200,000 nucleotides long. The G+C content is about 65%. The genome contains about 4000 genes. Genes that code for lipid metabolism are a very important part of the bacterial genome, and 8% of the genome is involved in this activity. The different species of the Mycobacterium tuberculosis complex show a 95-100% DNA relatedness based on studies of DNA homology, and the sequence of the 16S rRNA gene are exactly the same for all the species.

Intended Use

RevoDx MTBC qPCR Kit is an in vitro nucleic acid amplification test for qualitative detection of M. tuberculosis complex (M. tuberculosis, M. africanum, M. bovis, M. bovis BCG, M. canettii, M. microti, M. pinnipedii) DNA.