RevoDx Extended Spectrum Beta-Lactamases (ESBL) Genes (CTX-M, TEM, SHV) qPCR Kit

Beta-lactamases are enzymes that are major cause of bacterial resistance to the beta-lactam family of antibiotics such as penicillins, cephalosporins, cephamycins, and carbapenems. These enzymes catalyze the hydrolysis of theamide bond of beta-lactam ringand render the antibiotic inactive against its original cellular target, the cell wall transpeptidase. Extended-spectrum beta-lactam antibiotics have widely been used for treatment of serious Gram-negative infections. Fast and adequate detection of extended-spectrum β-lactamases (ESBLs) is crucial for infection control measures and the choice of antimicrobial therapy. The aim of this study was to develop and evaluate a novel ESBL assay using qPCR method to detect the most prevalent ESBLs in Enterobacteriaceae: TEM, SHV and CTX-M.

Intended Use

RevoDx Extended Spectrum Beta-Lactamases (ESBL) Genes qPCR Kitis a real-time PCR test intended for the qualitative detection of extended spectrum Beta-lactamasesgenes: CTX-M, TEM, SHVgenes.