RevoDx Epstein-Barr Virus qPCR Kit
Epstein-Barr virus (EBV), a gamma herpesvirus, is a ubiquitous cause of infection in up to 95% of the general population. EBV is a DNA virus with a toroid-shaped protein core that is wrapped with DNA, a nucleocapsid with 162 capsomers, a protein tegument between the nucleocapsid and the envelope, and an outer envelope with external virus-encoded glycoprotein spikes. The EBV genome is a linear, double-stranded, ~172-kb DNA molecule that encodes > 85 genes. EBV infects B lymphocytes leading to their immortalisation, with persistence of the EBV genome as an episome. The disease can last several weeks and is characterized by lymphocytosis, sore throat, lymphadenopathy, and fatigue. Exposure to oral secretions during deep kissing has been identified as the major source for primary EBV infection in adolescents. Furthermore EBV has been associated with a remarkably diverse range of cancer types. Because EBV persists in the B cells of the asymptomatic host, it can easily be envisaged how it contributes to the development of B-cell lymphomas. However, EBV is also found in other cancers, including T-cell/natural killer cell lymphomas and several epithelial malignancies. It is reported that 80-86% of adults in Turkey are seropositive to EBV.
Intended Use
RevoDx Epstein-Barr Virus qPCR Kit is an in vitro nucleic acid amplification test for quantification of Epstein-Barr Virus (EBV) DNA in human serum or plasma (EDTA) or cerebrospinal fluid (CSF) specimens.
Features
- Ready-to-use kit
- Quantification Standards ranging from 1 x 104 IU/mL to 1 x 107 IU/mL
- Exogenous internal control
- The Limit of Detection (LoD) value is 86 IU/mL
- Linear range is between 97 IU/mL and 1 x 109 IU/mL
- Diagnostic specificity is ≥ 99 %
- Whole system failure rate is ≤1 %
- CE-IVD marked