RevoDx Colistin (mcr) resistance qPCR Kit
Colistin is an antimicrobial agent of the polymyxin class. Due to the recent dissemination of multidrug resistant (MDR) bacteria around the world, colistin has been increasingly used as a last resort antimicrobial for treatment. It is a cationic polypeptide containing an acylated tripeptide chain at its N-terminus responsible for the toxicity of colistin. The mechanism of action relies on the interaction of the hydrophobic region of the fatty acid and phosphate groups of the lipid A of the lipopolysaccharide (LPS). This interaction displaces the divalent cations that naturally stabilize the outer bacterial membranes leading to leakage of cellular compounds and, ultimately, cell death. Due to its mechanism of action, colistin is highly effective against most Enterobacterales species and non-fermenting Gram-negative bacteria such as Acinetobacter baumannii and Pseudomonas aeruginosa. Conversely, colistin is not active against Gram-positive bacteria, Gram-negative cocci, and anaerobic bacteria.
Intended Use
RevoDx Colistin resistance qPCR Kit is a real-time PCR test intended for the qualitative detection of Colistin resistance gene (mcr).
Features
- Ready-to-use kit
- Compatible with most real-time PCR detection systems with the channels FAM and HEX
- Human RNase P as an internal control
- The Limit of Detection (LoD) value is 80 CFU/mL
- No cross-reactivity with the potential cross-reactive markers shown using both in silico analysis and wet testing.
- CE-IVD marked