RevoDx CMV qPCR Kit
Cytomegaloviruses (CMVs) belong to the betaherpesviruses subfamily of herpesviruses and are ubiquitous but highly species-specific viruses that infect many animals, including humans. Infection with CMV is common in all populations and rarely associated with symptomatic infection in normal hosts. In contrast, it is a major cause of multiorgan disease in immunocompromised patients. CMV is also a leading cause of congenital infection and a leading infectious cause of brain disease and hea-ring loss in children in the United States and western Europe. As with other herpesviruses, primary infection with CMV is followed by a persistent infection.
CMV is morphologically similar to other herpesviruses and is the largest member of the family. The virus consists of a 64-nm core enclosed by a 110-nm icosahedral capsid. The capsid is surrounded by a poorly defined amorphous tegument that itself is surrounded by a loosely applied, lipid-containing tegument. The genome of CMV consists of linear double-stranded deoxyribonucleic acid (DNA) molecule approx 240 kb. The genome of CMV is similar to that of herpes simplex virus in that it has long and short unique segments, both of which are bounded by homologous repetitive sequences. The CMV genome is approx 50% larger than herpes simplex virus and encodes for at least 35 struc-tural proteins and an undefined number of nonstructural proteins. Although the replication of CMV is very similar to that described for herpes simplex virus, the replicative cycle is much slower than for herpes simplex.
Intended Use
RevoDx CMV qPCR Kit is a real-time PCR test intended for quantification of Human Cytomegalovirus (CMV) DNA in human serum or plasma (EDTA)
Features
- Validated for use with fresh or frozen human serum or plasma collected in EDTA anticoagulant.
- Quantification Standards ranging from 1 x 104 IU/mL to 1 x 107 IU/mL
- Exogenous internal control
- The Limit of Detection (LoD) value is 56 IU/mL
- Linear range is between 71 IU/mL and 1 x 109 IU/mL
- Diagnostic specificity is ≥ 99 %
- Whole system failure rate is ≤1 %
- For research use only
RevoDx CMV Qualitative qPCR Kit
Cytomegaloviruses (CMVs) belong to the betaherpesviruses subfamily of herpesviruses and are ubiquitous but highly species-specific viruses that infect many animals, including humans. Infection with CMV is common in all populations and rarely associated with symptomatic infection in normal hosts. In contrast, it is a major cause of multiorgan disease in immunocompromised patients. CMV is also a leading cause of congenital infection and a leading infectious cause of brain disease and hea-ring loss in children in the United States and western Europe. As with other herpesviruses, primary infection with CMV is followed by a persistent infection.
CMV is morphologically similar to other herpesviruses and is the largest member of the family. The virus consists of a 64-nm core enclosed by a 110-nm icosahedral capsid. The capsid is surrounded by a poorly defined amorphous tegument that itself is surrounded by a loosely applied, lipid-containing tegument. The genome of CMV consists of linear double-stranded deoxyribonucleic acid (DNA) molecule approx 240 kb. The genome of CMV is similar to that of herpes simplex virus in that it has long and short unique segments, both of which are bounded by homologous repetitive sequences. The CMV genome is approx 50% larger than herpes simplex virus and encodes for at least 35 struc-tural proteins and an undefined number of nonstructural proteins. Although the replication of CMV is very similar to that described for herpes simplex virus, the replicative cycle is much slower than for herpes simplex.
Intended Use
RevoDx CMV Qualitative qPCR Kit is a real-time PCR test intended for the qualitative detection of Human Cytomegalovirus (CMV) DNA in human serum or plasma (EDTA)
Features
- Validated for use with fresh or frozen human serum or plasma collected in EDTA anticoagulant.
- Exogenous internal control
- The Limit of Detection (LoD) value is 56 IU/mL
- Diagnostic specificity is ≥ 99 %
- Whole system failure rate is ≤1 %